This rapid kit uses a fluorescence immunochromatography method to detect SARS-CoV-2 N antigen. The sample to be tested is applied to the sample window of the test cassette.
The SARS-CoV-2 N-antigen in the sample forms a complex with the antibody labeled with fluorescent microspheres. This complex migrates along the membrane and reaches the test region (T-line) on which a second antibody against the SARS-CoV-2 N-antigen is applied. Unbound fluorescent microspheres migrate along the membrane to the control region (C-line) and are bound by the control region anti-body. The test result in the test window is made visible with a UV lamp with a wavelength of 365 nm. If both the T-line and the C-line fluoresce, the test result is SARS-CoV-2 N-antigen positive; if only the C-line fluoresces and no T-line becomes visible, the test result is SARS-CoV-2 N-antigen negative. If no C-line becomes visible the test result is invalid and the sample must be retested with a new test cassette.